Antoni van Leewenhoek reported microscopic sperm morphology in 1678 for human and dog sperm ( Ruestow, 1983) using an early single-lens microscope ( Amann and Katz, 2004 Amann and Waberski, 2014). Microscopic assessment of sperm in motion has been used as a clinical and research tool for human and animal reproductive health over the past 340 years. The results indicate that the iSperm application offers an accurate and alternative measurement of motility to traditional CASA analysis, though caution should be taken when assessing concentration due to the high CV observed in this study. For iSperm assessment of total and progressive motility, the CVs were 6.3 ± 0.5% and 10.7 ± 0.8%, respectively. For fresh and frozen-thawed samples, concentration assessment by iSperm showed high variability (CV= 19.9 ± 1.5%). We also determined the coefficient of variation (CV) for repeatability of sample analysis for iSperm and CASA for fresh sperm, wherein each sample was assessed 10 times on both devices. Significant positive correlations were found between CASA assessment and iSperm for both progressive and total motility measurements. Spearman’s Rho correlational analysis was used to identify significant associations between motility assessment methods. The experiment used fresh and frozen/thawed canine semen samples for comparisons of semen analysis parameters (concentration, total motility, and progressive motility) between a CASA system, iSperm, and NucleoCounter SP-100 (concentration) instruments. The objective of this study was to evaluate the repeatability and accuracy of canine sperm motility (total and progressive) assessment with a tablet-based Canine iSperm instrument compared to computer-assisted sperm analysis (CASA).
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